Bacteriospermia assessment and its relationship with conventional seminal parameters in stud dogs ejaculates (Canis familiaris)

The objective of this work was to determine the frequency bacteriospermia and its effect on seminal quality in canines. Dogs were divided into two groups according weight: small dogs between 1 10 kg (Group 1) with more than 2). foreskin disinfected each animal (n=15), semen sample collected by gloved hand method. Sperm motility, morphology, viability, concentration evaluated using a 40x microscope. A 10µL drop cultured diffusion method blood agar MacConkey agar. Colony-forming units (CFU) quantitatively evaluated, biochemical identification carried out after 48 hours at 37°C. Conventional PCR performed samples evaluate presence/absence 11 bacteria. Bacterial growth found all samples. CFU/mL 34042.8 for group 107714.3 2, while 142.9 CFU/mL, 21328.6 respectively. Coagulase-negative Staphylococcus most frequent bacteria isolated conventional culture (64.3%), aureus Klebsiella spp. common PCR. Between both groups, only statistically significant difference normal sperm morphology. negative correlation observed viability morphology CFU One dog ruled because he had azoospermia. Canine is quite could alter quality. presence Gram-negative associated greater alteration analysis.